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1.  Skin regeneration stimulation: the role of PCLplatelet gel nanofibrous scaffold
Parviz Ranjbarvan, Masoud Soleimani, Ali Samadi Kuchaksaraei, Jafar Ai, Reza Faridi Majidi, Javad Verdi
Journal Paper
Microsc Res Tech. 2017 May;80(5):495-503


Skin is the largest organ of the human body. Thus far, tissue engineering of skin has developed rapidly and has used many types of growth factors and nanofibrous scaffolds. In this study, we differentiated neonate keratinocytes for epithelialization on the polycaprolactone-Platelet gel (PCL-PG) scaffold. Fabricated PCL nanofibers prepared by electrospinning technology and coated by platelet gel. Subsequently, the structure of the scaffold was evaluated by SEM, FTIR-ATR, contact angle and tensile test assays. After seeding the neonate keratinocytes on neat PCL and PCL-PG scaffolds, the epidermal maturation was tested by detecting cytokeratin 10 and loricrin determinants by immunocytochemistry; moreover, keratinocyte genes such as keratin 14, keratin 10, and Involucrin were investigated by real-time PCR. The results of MTT assay indicated an increase in cell viability and cell proliferation of neonate keratinocytes on PCL-PG nanofiber scaffolds compared with PCL. RT-PCR and immunocytochemical analysis showed better cell differentiation on the PCL-PG scaffolds than neat PCL. Furthermore, SEM microscopy images demonstrated that neo-keratinocytes enhance adhesion and proliferation on PCL-PG nanofiber scaffolds. We found that PG increases biocompatibility and wettability of scaffold, cell adhesion, and expression of keratinocyte markers. Overall, this procedure is recommended to be employed in skin tissue engineering and wounds healing.

2.  The Development of an Empirical Model for Estimation of the Sensitivity to Heat Stress in the Outdoor Workers at Risk
M Asghari , P Nassiri ,MR Monazzam , F Golbabaei ,H Arabalibeik ,A Shamsipour
Journal Paper
Annals of Medical and Health Sciences, Research , March-April 2017, Vol 7, Issue 2


Background: Workers who work in hot environments may be at risk for heat stress. Exposure to heat can result in occupational illnesses, including heat stroke, heat cramps, and heat exhaustion. The risk of exposure to heat depends on individual, environmental, and occupational risk factors. Individual risk factors may decrease the individual’s tolerance to heat stress. Sensitivity as an intrinsic factor may predispose a person to heat stress. Aim: This study was aimed to determine the criteria for sensitivity parameter, specify their weights using the fuzzy Delphi-analytical hierarchy, and finally providing a model to estimate sensitivity. The significant of the study is presenting a model to estimate the sensitivity to heat stress. Subjects and Methods: The expert’s opinions were used to extract the criteria in Delphi method. After determining the weight of each criterion, Fuzzy analytic hierarchy Process (FAHP), by mathematical principles matrix and triangular fuzzy numbers, was applied for the prioritization of criteria. Results: According to experts’ viewpoints and considering some exclusion, 10 of 36 criteria were selected. Among 10 selected criteria, age had the highest percentage of responses (90% (27/30)) and its relative weight was 0.063. After age, the highest percentages of response were assigned to the factors of preexisting disease (66.6% (20/30)), body mass index (56.6% (17/30)), work experience (53.3% (16/30)), and clothing (40% (16/30)), respectively. Other effective criteria on sensitivity were metabolic rate, daily water consumption, smoking habits, drugs that interfere with the thermoregulatory processes, and exposure to other harmful agents. Conclusions: Eventually, based on the criteria, a model for estimation of the workers’ sensitivity to heat stress was presented for the first time, by which the sensitivity is estimated in percent. 

3.  Determination and weighting the effective criteria in selecting a heat stress index using the Delphi echnique and fuzzy analytic hierarchy process (FAHP)
Mahdi Asghari , Parvin Nassiri , Mohammad Reza Monazzam , Farideh Golbabaei , Hossein Arabalibeik , Ali Akbar Shamsipour
Journal Paper
JHSW 2017, 7(1): 23-32


Introduction: To evaluate heat stress in the workplace different indices are used. But, none of these measures have been widely accepted. The aim of this study was to determine effective criteria in selecting a heat stress index and weighting of these criteria.
Material and Method: This study is a qualitative study that was designed and conducted within 10 stages. In the current study, effective criteria for choosing a heat stress index was determined by using Delphi technique and expert's opinions (30 people), and then the fuzzy analytic hierarchy process was used for weighting and prioritization of these criteria.
Results: Analyzing the completed questionnaires by the experts and according to the percentage of respondents, 11  criteria were finally selected. "Simplicity" criteria by %76.6 had the highest percentage of response. But, the relative weight of this criteria was 0.041, and had the lowest weight after the "low cost" criteria. The criteria relating to the "Standard" item was 0.141as the highest
relative weight.

4.  Personal risk factors during heat stress exposure in workplace
Parvin Nassiri . Mohammad Reza Monazzam , Farideh Golbabaei , Aliakbar Shamsipour , Hossein Arabalibeik , Marzieh Abbasinia , Masoumeh Chavoshi , Mehdi Asghari
Journal Paper
JHSW 2017, 7(2): 163-180


Introduction: Heat stress is caused by many factors such as individual factors, environmental factors, and management factors. Individual risk factors can decrease the heat tolerance, and play an important role in heat stress disorders incidence. The aim of this study was to review individual parameters influencing the thermal stress and review the preceding studies.
Material and Method: In this review study sites such as Web of Science, Scopus, Pubmed, Iran Medex, Magiran, Google Scholar and SID databases were used for search. The keywords included heat stress, personal factors and heat exposure. The period of 1995 to 2015 was investigated, and finally 75 original articles were identified.
Result: At the individual level, exposure with a single risk factor may reduce the workers' heat tolerance; while exposure with a combination of several risk factors probably synergistically increases the risk of heatrelated disorders. Individual risk factors include age, gender, obesity, fatigue, race, and previous heat disorders and dehydration. In addition, some diseases (such as cardiovascular disease, diabetes and infectious diseases) or use of certain drugs or alcohol can reduce the heat tolerance.

5.  Graphene/cobalt nanocarrier for hyperthermia therapy and MRI diagnosis
S Hatamie ,MM Ahadian , MA Ghiass ,A Iraji Zad ,R Saber ,B Parseh ,MA Oghabian ,S Shanehsazzadeh
Journal Paper
Colloids Surf B Biointerfaces.


Graphene/cobalt nanocomposites are promising materials for theranostic nanomedicine applications, which are defined as the ability to diagnose, provide targeted therapy and monitor the response to the therapy. In this study, the composites were synthesized via chemical method, using graphene oxide as the source material and assembling cobalt nanoparticles of 15 nm over the surface of graphene sheets. Various characterization techniques were then employed to reveal the morphology, size and structure of the nanocomposites, such as X-ray diffraction analysis, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, high resolution transmission electron microscopy and ultraviolet visible spectroscopy. Using ion-coupled plasma optical emission spectroscopy, cobalt concentration in the nanocomposites was found to be 80%. In addition, cytotoxicity of graphene/cobalt nanocomposites were evaluated using 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyltetrazolium bromide or MTT assay. MTT viability assay exhibited biocompatibility to L929 mouse fibroblasts cells, under a high dose of 100 μg/mL over 24 h. Hyperthermia results showed the superior conversion of electromagnetic energy into heat at 350 kHz frequency for 0.01 and 0.005 g/L of the nanocomposites solution. The measured heat generation and energy transfer results were anticipated by the finite element analysis, conducted for the 3D structure. Magnetic resonance imaging characteristics also showed that negatively charge graphene/cobalt nanocomposites are suitable for T1-weighted imaging.

6.  Detecting Driver Mental Fatigue Based on EEG Alpha Power Changes during Simulated Driving
F Gharagozlou ,G Nasl Saraji ,A Mazloumi ,A Nahvi , A Motie Nasrabadi ,A Rahimi Foroushani ,A Arab Kheradmand ,M Ashouri ,M Samavati
Journal Paper
Iran J Public Health. 2015 Dec;44(12):1693-700



Driver fatigue is one of the major implications in transportation safety and accounted for up to 40% of road accidents. This study aimed to analyze the EEG alpha power changes in partially sleep-deprived drivers while performing a simulated driving task.


Twelve healthy male car drivers participated in an overnight study. Continuous EEG and EOG records were taken during drivingon a virtual reality simulator on a monotonous road. Simultaneously, video recordings from the driver face and behavior were performed in lateral and front views and rated by two trained observers. Moreover, the subjective self-assessment of fatigue was implemented in every 10-min interval during the driving using Fatigue Visual Analog Scale (F-VAS). Power spectrum density and fast Fourier transform (FFT) were used to determine the absolute and relative alpha powers in the initial and final 10 minutes of driving.


The findings showed a significant increase in the absolute alpha power (P = 0.006) as well as F-VAS scores during the final section of driving (P = 0.001). Meanwhile, video ratings were consistent with subjective self-assessment of fatigue.


The increase in alpha power in the final section of driving indicates the decrease in the level of alertness and attention and the onset of fatigue, which was consistent with F-VAS and video ratings. The study suggested that variations in alpha power could be a good indicator for driver mental fatigue, but for using as a countermeasure device needed further investigations.

7.  Differentiation of human endometrial stem cells into urothelial cells on a three-dimensional nanofibrous silk–collagen scaffold: an autologous cell resource for reconstruction of the urinary bladder wall
Alireza Shoae-Hassani, Seyed Abdolreza Mortazavi-Tabatabaei, Shiva Sharif, Alexander Marcus Seifalian, Alireza Azimi, Ali Samadikuchaksaraei, Javad Verdi
Journal Paper


Reconstruction of the bladder wall via in vitro differentiated stem cells on an appropriate scaffold could be used in such conditions as cancer and neurogenic urinary bladder. This study aimed to examine the potential of human endometrial stem cells (EnSCs) to form urinary bladder epithelial cells (urothelium) on nanofibrous silkcollagen scaffolds, for construction of the urinary bladder wall. After passage 4, EnSCs were induced by keratinocyte growth factor (KGF) and epidermal growth factor (EGF) and seeded on electrospun collagenV, silk and silkcollagen nanofibres. Later we tested urotheliumspecific genes and proteins (uroplakinIa, uroplakinIb, uroplakinII, uroplakinIII and cytokeratin 20) by
immunocytochemistry, RTPCR and western blot analyses. Scanning electron microscopy (SEM) and histology were used to detect cellmatrix interactions. DMEM/F12 supplemented by KGF and EGF induced EnSCs to express urothelial cellspecific genes and proteins. Either collagen, silk or silkcollagen scaffolds promoted cell proliferation. The nanofibrous silkcollagen scaffolds provided a threedimensional (3D) structure to maximize cellmatrix penetration and increase differentiation of the EnSCs. Human EnSCs seeded on 3D nanofibrous silkcollagen scaffolds and differentiated to urothelial cells provide a suitable source for potential use in bladder wall reconstruction in women.
Copyright (C) 2013 John Wiley & Sons, Ltd.

8.  Optimizing parameters on alignment of PCL/PGA nanofibrous scaffold: An artificial neural networks approach
Farnoush Asghari Paskiabi , Esmaeil Mirzaei , Amir Amani , Mohammad Ali Shokrgozar , Reza Saber , Reza Faridi-Majidi
Journal Paper
International Journal of Biological Macromolecules 81 (2015) 1089–1097


This paper proposes an artificial neural networks approach to finding the effects of electrospinning parameters on alignment of poly(epsiloncaprolactone)/ poly(glycolic acid) blend nanofibers. Four electrospinning parameters, namely total polymer concentration, working distance, drum speed and applied voltage were considered as input and the standard deviation of the angles of nanofibers, introducing fibers alignments, as the output of the model. The results demonstrated that drum speed and applied voltage are two critical factors influencing nanofibers alignment, however their effect are entirely interdependent. Their effects also are not independent of other electrospinning parameters. In obtaining aligned electrospun nanofibers, the concentration and working distance can also be effective. In vitro cell culture study on random and aligned nanofibers showed directional growth of cells on aligned fibers. (C) 2014 Elsevier B.V. All rights reserved

9.  Analytical Characterization of LabelFree Immunosensor Subsystems Based on MultiWalled Carbon Nanotube ArrayModified Gold Interface
Masoumeh Zahmatkeshan, Hoda Ilkhani, Malihe Paknejad, Moein Adel1,, Saeed Sarkarand, Reza Saber
Journal Paper
Combinatorial Chemistry & High Throughput Screening


In the present work, we report characterization studies of antigen(Ag)antibody (Ab) interaction based on the multiwalled carbon nanotube arraymodified gold electrode for labelfree electrochemical immunosensor. COOH functionalization of MWCNT was suggested by FTIR spectroscopy. Images from atomic force microscopy (AFM) and RAMAN spectroscopy confirmed the interaction of Ab on the gold interface based on the MWCNT array. A cyclic voltammetric study exhibited a linear response in phosphate buffered saline (PBS) solution including 1mM K3Fe (CN) 6 at pH 7.4 with PSA concentrations over a range of 2 to 40 ngmL(1), and a calculated detection limit of 0.56 ngmL(1).

10.  Functionalization of PANBased Electrospun Carbon Nanofibers by Acid Oxidation: Study of Structural, Electrical and Mechanical Properties
E Mirzaei, J Ai, M Sorouri, H Ghanbari,J Verdi,R FaridiMajidi
Journal Paper
Fullerenes, Nanotubes and Carbon Nanostructures


Electrospun carbon nanofibers (ECNFs) have many qualities that make them attractive for applications in various fields of technology. For many applications, it is necessary to modify the surface of CNFs by chemical functionalization. Here, a systematic functionalization of polyacrylonitrile (PAN)-based ECNFs by oxidation in concentrated nitric acid was examined. The effects of oxidation on the microstructural, electrical, and mechanical characteristics have been studied. The Fourier transform infrared spectroscopy results revealed the time-dependent functionalization of ECNFs. However, according to the scanning electron microscopy, there was no considerable change in the surface morphology of CNFs due to functionalization. The microstructures as well as electrical and mechanical properties were severely dependent on oxidation duration according to X-ray diffraction and Raman spectroscopies as well as conductivity and mechanical strength measurements, respectively. Increase in oxidation duration led to more structural disorders and noticeable decrease in electrical conductivity and mechanical strength.


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