Microsc Res Tech. 2017 May;80(5):495-503 Skin regeneration stimulation: the role of PCLplatelet gel nanofibrous scaffold Author(s): Parviz Ranjbarvan, Masoud Soleimani, Ali Samadi Kuchaksaraei, Jafar Ai, Reza Faridi Majidi, Javad Verdi
Abstract
Skin is the largest organ of the human body. Thus far, tissue engineering of skin has developed rapidly and has used many types of growth factors and nanofibrous scaffolds. In this study, we differentiated neonate keratinocytes for epithelialization on the polycaprolactone-Platelet gel (PCL-PG) scaffold. Fabricated PCL nanofibers prepared by electrospinning technology and coated by platelet gel. Subsequently, the structure of the scaffold was evaluated by SEM, FTIR-ATR, contact angle and tensile test assays. After seeding the neonate keratinocytes on neat PCL and PCL-PG scaffolds, the epidermal maturation was tested by detecting cytokeratin 10 and loricrin determinants by immunocytochemistry; moreover, keratinocyte genes such as keratin 14, keratin 10, and Involucrin were investigated by real-time PCR. The results of MTT assay indicated an increase in cell viability and cell proliferation of neonate keratinocytes on PCL-PG nanofiber scaffolds compared with PCL. RT-PCR and immunocytochemical analysis showed better cell differentiation on the PCL-PG scaffolds than neat PCL. Furthermore, SEM microscopy images demonstrated that neo-keratinocytes enhance adhesion and proliferation on PCL-PG nanofiber scaffolds. We found that PG increases biocompatibility and wettability of scaffold, cell adhesion, and expression of keratinocyte markers. Overall, this procedure is recommended to be employed in skin tissue engineering and wounds healing.
Parviz Ranjbarvan, Masoud Soleimani, Ali Samadi Kuchaksaraei, Jafar Ai, Reza Faridi Majidi, Javad Verdi, Skin regeneration stimulation: the role of PCLplatelet gel nanofibrous scaffold Microsc Res Tech. 2017 May;80(5):495-503
M Asghari , P Nassiri ,MR Monazzam , F Golbabaei ,H Arabalibeik ,A Shamsipour , The Development of an Empirical Model for Estimation of the Sensitivity to Heat Stress in the Outdoor Workers at Risk Annals of Medical and Health Sciences, Research , March-April 2017, Vol 7, Issue 2
Mahdi Asghari , Parvin Nassiri , Mohammad Reza Monazzam , Farideh Golbabaei , Hossein Arabalibeik , Ali Akbar Shamsipour , Determination and weighting the effective criteria in selecting a heat stress index using the Delphi echnique and fuzzy analytic hierarchy process (FAHP) JHSW 2017, 7(1): 23-32